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normal human a b serum  (Thermo Fisher)


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    Structured Review

    Thermo Fisher normal human a b serum
    Normal Human A B Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+a+b+serum/HUMAN+SERUM+TYPE+AB/pm38629062-379-20-29
    Average 99 stars, based on 1 article reviews
    normal human a b serum - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Recombinant:

    Article Title: Clonal structure and the specificity of vaccine-induced T cell response to SARS-CoV-2 Spike protein
    Article Snippet: Measurement of IFNγ secretion in CD4 + and CD8 + T cells was performed using the IFN-γ secretion assay-detection kit (Miltenyi Biotec; 130-090-762) according to the manufacturer’s protocol. .. Briefly, after expansion with recombinant SARS-CoV-2 Spike protein, cells were pooled and resuspended in RPMI 1640 culture medium (GIBCO) supplemented with 5% normal human A/B serum and 1 mM sodium pyruvate (GIBCO) and plated at a density of 1-10 × 10 6 cells/ml. .. Cells were treated with 20 μg/mL recombinant SARS-CoV-2 Spike protein for 16 hours and with cells from the same donor frozen on day 0 at a ratio of 1:2-1:4 used as feeder, followed by incubation with IFNγ Catchmatrix reagent (Miltenyi Biotec; 130-090-762) for 5 minutes at 4°C.

    Article Title: Clonal structure and the specificity of vaccine-induced T cell response to SARS-CoV-2 Spike protein.
    Article Snippet: 4.7 IFNg-secretion assay Measurement of IFNg secretion in CD4+ and CD8+ T cells was performed using the IFN-g secretion assay-detection kit (Miltenyi Biotec; 130-090-762) according to the manufacturer’s protocol. .. Briefly, after expansion with recombinant SARS-CoV-2 Spike protein, cells were pooled and resuspended in RPMI 1640 culture medium (GIBCO) supplemented with 5% normal human A/B serum and 1 mM sodium pyruvate (GIBCO) and plated at a density of 1-10 × 106 cells/ml. .. Cells were treated with 20 mg/mL recombinant SARS-CoV-2 Spike protein for 16 hours and with cells from the same donor frozen on day 0 at a ratio of 1:2-1:4 used as feeder, followed by incubation with IFNg Catchmatrix reagent (Miltenyi Biotec; 130-090-762) for 5 minutes at 4°C.

    Isolation:

    Article Title: Clonal structure and the specificity of vaccine-induced T cell response to SARS-CoV-2 Spike protein
    Article Snippet: .. Freshly isolated PBMCs were plated in a 96-well plate (Sarstedt; 83.3925.500) at a density of 1.5 × 10 6 cells/well in RPMI 1640 culture medium (Thermo; 31870025) supplemented with 1 mM sodium pyruvate (Thermo; 11360039), 5% normal human A/B serum, 1 mM L-glutamine (Thermo; 25030-024) and SARS-CoV-2 S protein-derived peptide pools (Miltenyi Biotec; 130-126-701) (final concentration 1 mM) followed by 1h incubation 37°C, 5% СО2. .. GolgiPlug (BD Biosciences; 555028) was added for 5h incubation (37°С, 5% СО2).

    Article Title: Clonal structure and the specificity of vaccine-induced T cell response to SARS-CoV-2 Spike protein.
    Article Snippet: .. Freshly isolated PBMCs were plated in a 96-well plate (Sarstedt; 83.3925.500) at a density of 1.5 × 106 cells/well in RPMI 1640 culture medium (Thermo; 31870025) supplemented with 1 mM sodium pyruvate (Thermo; 11360039), 5% normal human A/B serum, 1 mM L-glutamine (Thermo; 25030-024) and SARS-CoV2 S protein-derived peptide pools (Miltenyi Biotec; 130-126-701) (final concentration 1 mM) followed by 1h incubation 37°C, 5% СО2. .. GolgiPlug (BD Biosciences; 555028) was added for 5h incubation (37°С, 5% СО2).

    Concentration Assay:

    Article Title: Clonal structure and the specificity of vaccine-induced T cell response to SARS-CoV-2 Spike protein
    Article Snippet: .. Freshly isolated PBMCs were plated in a 96-well plate (Sarstedt; 83.3925.500) at a density of 1.5 × 10 6 cells/well in RPMI 1640 culture medium (Thermo; 31870025) supplemented with 1 mM sodium pyruvate (Thermo; 11360039), 5% normal human A/B serum, 1 mM L-glutamine (Thermo; 25030-024) and SARS-CoV-2 S protein-derived peptide pools (Miltenyi Biotec; 130-126-701) (final concentration 1 mM) followed by 1h incubation 37°C, 5% СО2. .. GolgiPlug (BD Biosciences; 555028) was added for 5h incubation (37°С, 5% СО2).

    Article Title: Clonal structure and the specificity of vaccine-induced T cell response to SARS-CoV-2 Spike protein.
    Article Snippet: .. Freshly isolated PBMCs were plated in a 96-well plate (Sarstedt; 83.3925.500) at a density of 1.5 × 106 cells/well in RPMI 1640 culture medium (Thermo; 31870025) supplemented with 1 mM sodium pyruvate (Thermo; 11360039), 5% normal human A/B serum, 1 mM L-glutamine (Thermo; 25030-024) and SARS-CoV2 S protein-derived peptide pools (Miltenyi Biotec; 130-126-701) (final concentration 1 mM) followed by 1h incubation 37°C, 5% СО2. .. GolgiPlug (BD Biosciences; 555028) was added for 5h incubation (37°С, 5% СО2).

    Incubation:

    Article Title: Clonal structure and the specificity of vaccine-induced T cell response to SARS-CoV-2 Spike protein
    Article Snippet: .. Freshly isolated PBMCs were plated in a 96-well plate (Sarstedt; 83.3925.500) at a density of 1.5 × 10 6 cells/well in RPMI 1640 culture medium (Thermo; 31870025) supplemented with 1 mM sodium pyruvate (Thermo; 11360039), 5% normal human A/B serum, 1 mM L-glutamine (Thermo; 25030-024) and SARS-CoV-2 S protein-derived peptide pools (Miltenyi Biotec; 130-126-701) (final concentration 1 mM) followed by 1h incubation 37°C, 5% СО2. .. GolgiPlug (BD Biosciences; 555028) was added for 5h incubation (37°С, 5% СО2).

    Article Title: Clonal structure and the specificity of vaccine-induced T cell response to SARS-CoV-2 Spike protein.
    Article Snippet: .. Freshly isolated PBMCs were plated in a 96-well plate (Sarstedt; 83.3925.500) at a density of 1.5 × 106 cells/well in RPMI 1640 culture medium (Thermo; 31870025) supplemented with 1 mM sodium pyruvate (Thermo; 11360039), 5% normal human A/B serum, 1 mM L-glutamine (Thermo; 25030-024) and SARS-CoV2 S protein-derived peptide pools (Miltenyi Biotec; 130-126-701) (final concentration 1 mM) followed by 1h incubation 37°C, 5% СО2. .. GolgiPlug (BD Biosciences; 555028) was added for 5h incubation (37°С, 5% СО2).



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